NIV Congres

Introduction AQGV previously described by Khan et al has been utilized as an anti-inflammatory molecule. This new molecule showed promising results in septic and shock inflammatory models. Brain death (BD) is an inflammatory condition, which is deleterious for organ quality in transplantation. Therefore, we aim to assess the effect of AQGV in a brain death rat model.

Method BD was induced in rats by inflating a subdurally placed balloon catheter. Animals were treated with PBS or AQGV (30 mg/kg) one hour before BD. After 4 hours of BD, serum, kidneys and livers were collected. Sham-operated rats treated with PBS or AQGV served as controls. Tissue gene expression was measured by Real Time qPCR. Tissue protein expression was detected by immunohistochemical analyses.

Results After the BD period, plasma levels of IL-6, creatinine, AST, ALT and LDH were not significantly reduced after AQGV treatment. Polymorphonuclear influx in liver and kidney tissue were not reduced. Relative expression of inflammatory genes (IL-6, TNF-a, MCP-1 and C3) were not significantly down-regulated in liver and kidney.

Discussion Pre-treatment with AQGV in this model did not show any anti-inflammatory effects. However, this could be attributed to timing or dosing. We postulate that AQGV could exert anti-inflammatory effects when administered after brain death induction based on the chemical properties.